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Laboratory Safety
National Summary

This topic covers OSHA’s requirements for laboratory safety.

Because laboratories differ from industrial operations, particularly in their use and handling of hazardous chemicals, OSHA has a different set of requirements for laboratories than other facilities. The most important piece of OSHA’s Occupational Exposure to Chemicals in Laboratories standard is a requirement for laboratories to produce a chemical hygiene plan that establishes standard operating procedures for handling hazardous chemicals.

Components of a chemical hygiene plan include procedures for reducing employee exposure to hazardous chemicals (e.g., engineering controls, ventilation, and use of PPE), employee training, emergency measures, provisions for medical consultation and examination of workers, requirements for approval of new lab activities, and special precautions for working with particularly hazardous substances.

Click here for a chemical hygiene plan that you can customize for your facility.

For related resources and training materials, refer to the following topics:


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Directives 11/18/1991 - CPL 2.35 CH-19 - Changes to the Regulatory and General Industry Standard Alleged Violation... 07/22/2003National
Regulations 29 CFR § 1910.1450 Occupational exposure to hazardous chemicals in laboratories  National
Proposed Rules 29 CFR 1910 - Nationally Recognized Testing Laboratories; Proposed Rule 12/07/2009National
Final Rules 29 CFR 1910 - Occupational Exposure to Hazardous Chemicals in Laboratories 01/21/2013National
Notices Agency Information Collection Activities; Occupational Exposure to Hazardous Chemicals in Laboratories Standard 02/02/2012National
Notices Application of TUV Rheinland of North America, Inc. for Expansion of Recognition as Nationally Recognized Testing Laboratory 07/24/2012National
Questions & Answers Can you please tell me if we need to install an oxygen sensor in a cleanroom space that we are currently using dewars of nitrogen in? The two labs are 70 feet by 40 feet and 78 feet by 38 feet and they are connected by an opening between the two rooms... 08/22/2013National
Training Activities Chemical Hygiene Safety Activity (Word)  National
Trainer's Outlines Chemical Hygiene: What Your Chemical Hygiene Plan Must Include to Ensure Safety & Legal Compliance  National
Guidance Documents Chemical Hygiene: What Your Chemical Hygiene Plan Must Include to Ensure Safety & Legal Compliance  National
Trainer's Outlines Chemical Hygiene: What Your Chemical Hygiene Plan Must Include to Ensure Safety and Legal Compliance  National
Guidance Documents Chemical Hygiene: What Your Chemical Hygiene Plan Must Include to Ensure Safety and Legal Compliance  National
Notices Hazardous Chemicals in Laboratories Standard; Extension of the Office of Management and Budget's Approval of Information Collection Requirements 12/27/2004National
Notices Information collection activity: Regulation on Definition and Requirements for a Nationally Recognized Testing Laboratory 10/02/2013National
Checklists Lab Safety (PDF)  National
Handouts Lab Safety (PDF)  National
Quizzes Lab Safety (PDF)  National
Trainer's Outlines Lab Safety (PDF) [Safety Audit Checklists]  National
Plans Laboratory Chemical Hygiene Plan (29 CFR 1910.1450)  National
Audio Presentations Laboratory Safety  National
Handouts Laboratory Safety (PDF)  National
Quizzes Laboratory Safety (PDF)  National
Handouts Laboratory Safety (PDF)  National
Trainer's Guides Laboratory Safety (PDF)  National
Quizzes Laboratory Safety (PDF)  National
Handouts Laboratory Safety (PDF)  National
Quizzes Laboratory Safety (PDF) -- Spanish  National
Handouts Laboratory Safety (PDF) -- Spanish  National
Trainer's Outlines Laboratory Safety (PDF) [7-Minute Safety Trainer] (short outline)  National
Trainer's Outlines Laboratory Safety (PDF) [OSHA Training System] (long outline)  National
PowerPoints Laboratory Safety (PPT)  National
Handouts Laboratory Safety (Word)  National
Meetings Laboratory Safety (Word)  National
Quizzes Laboratory Safety (Word)  National
Training Activities Laboratory Safety Activity (Word)  National
Training Exercises Laboratory Safety Exercise 1 (PDF)  National
Training Exercises Laboratory Safety Exercise 2 (PDF)  National
Guidance Documents Laboratory Safety Guidance (OSHA document)  National
Quizzes Laboratory Safety Quiz (Word)  National
Speaker's Notes Laboratory Safety Trainer's Outline - Script View  National
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